Welcome to Barrett’s lab protocols documentation!¶ Bioanalyser Agilent Some additions to the protocol Maintenance Chip Priming station Chip tricks! Reagents DNA extractions Anolis Day 1: Tissue collection and digestion Materials Reagents Day 2: DNA Isolation – Step 1 Materials Reagents Day 3: DNA Isolation – Step 2 Materials Reagents Solutions Pocillopora Reagents In advance Day 1 Day 2 Heliconius Materials Reagents Preparation 1 Preparation 2 Preparation 3 Solutions Finch Materials Day 1: Tissue collection and digestion Day 2: DNA Isolation – Step 1 Day 3: DNA Isolation – Step 2 Storage Solutions References Stickleback Day 1: Tissue collection and digestion Day 2: DNA Isolation – Step 1 Day 3: DNA Isolation – Step 2 Solutions Lab material Micropipettor Directions Tissue sampling for RNA-seq Fish Lethal tissue sampling For non-lethal tissue sampling Reference